A Homogeneous HLA-B*27 Genotyping Assay Using Dried Reagent Mixtures
نویسندگان
چکیده
منابع مشابه
A Homogeneous HLA-B*27 Genotyping Assay Using Dried Reagent Mixtures
The presence of HLA-B*27 allele with patients suspected with ankylosing spondylitis can be used in the diagnostic process. We have developed an assay for typing for the HLA-B*27 in whole blood dried on sample collection cards using pre-dried reagent wells and homogeneous time-resolved fluorescence based PCR approach. Essentially only the sample needs to be added to the dry ready-to-use reaction...
متن کاملHLA-B27: what's new?
The HLA-B27 molecule is one of the most fascinating in medicine. Its contribution to the aetiopathogenesis of SpA and other diseases, and its protective action in certain infections, continue to challenge our understanding of its immunobiology and physiological roles. Animal studies have helped to cast light on ways in which HLA-B27 exerts its effects. Subtle variations in structure and behavio...
متن کاملHLA-B27 typing in the categorisation of uveitis in a HLA-B27 rich population.
AIMS To determine whether HLA-B27 typing helps the clinician in the diagnostic examination of uveitis in a HLA-B27 rich population and also whether the clinical picture of HLA-B27 positive unilateral acute or recurrent anterior uveitis (AAU) is distinguishable from the idiopathic negative form. METHODS During a 3 year period 220 consecutive patients with undetermined uveitis at onset were exa...
متن کاملHLA class II genotyping: two assay systems compared.
In the last few years, a variety of DNA-based human leukocyte antigen (HLA) typing methods have emerged, revealing the extreme polymorphism of HLA genes. This polymorphism makes it difficult for a clinical laboratory to establish the best HLA typing strategy. In this study we have compared two techniques for performing HLA-DRB typing: a commercial rapid assay based on the polymerase chain react...
متن کاملHLA-B27 and Ankylosing Spondylitis
Ankylosing spondylitis (AS) is a chronic inflammatory disease with potential disabling outcomes. Clinically, patients presented with inflammatory lower back pain, enthesis and alternated buttock pain (van der Linden & van der Heijde, 1998). Bernard Corner (16661698) was the first physician who published the clinical features of AS in his medical thesis (Baker & Weisman, 2006). In the late 19th ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Disease Markers
سال: 2009
ISSN: 0278-0240,1875-8630
DOI: 10.1155/2009/849784